| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1509-1 | ||||
Resumo:In dogs, Staphylococcus pseudintermedius strains most often cause skin and ear infections, and is also a potential zoonotic agent, which raises great concern for human health if these isolates are identified as multidrug resistant (MDR) or carriers of the resistance genes, such as methicillin-resistant Staphylococcus pseudintermedius (MRSP). Therefore, the objective of this study was to perform the molecular identification of S. pseudintermedius and to investigate patterns of antimicrobial and methicillin resistance in clinical isolates in dogs from Campo Grande - MS. The samples used in these studies come from the bacterial bank of the Laboratório de Bacteriologia of the Faculdade de Medicina Veterinária e Zootecnia da Universidade Federal de Mato Grosso do Sul (SISGEN C69392). Samples of bacteria isolated from dogs were selected in clinical cases of pyoderma and/or otitis, classified as Gram-positive cocci and catalase-producing. The samples were reactivated in Brain Heart Infusion (BHI) broth, analyzed for purity on BHI agar and the colonies were selected for antibiogram and DNA extraction by boiling. The antibiogram was carried out using the disk diffusion method, following the recommendations of the Brazilian Committee on Antimicrobial Susceptibility Testing (BrCAST), using six different classes of antimicrobials (aminoglycosides, beta-lactams, fluoroquinolones, folate inhibitors, macrolides and tetracycline), and samples with resistance to three or more different classes of antimicrobials, were classified MDR. Polymerase Chain Reaction (PCR) were performed to identify S. pseudintermedius, using the thermonuclease (nuc) gene and also to search for MRSP using the mecA gene. Thirty-nine samples were identified by PCR as S. pseuditermedius, of which 23 isolates (59%) were resistant to amyglycosides, 15 isolates (38.5%) were resistant to beta-lactams, 24 isolates (61.5%) were resistant to macrolides, 33 isolates (84.6%) were resistant to flourquinolones, 34 isolates (87.2%) for folate inhibitors, 37 isolates (94.9%) for tetracyclines, and 38 (97.4%) of these isolates classified as MDR. The mecA gene was not identified in any of the isolates. Although the samples do not present a resistance gene for mecA, the antimicrobial susceptibility pattern of the isolates, characterized by isolates with resistance to multiple antimicrobials, reinforces the concern about the indiscriminate use of antimicrobials, both from the perspective of animal health, as it restricts the possibility of effective treatment, in addition to the concern for One health, as animals can act as carriers of bacteria for human infections. Through this study it is possible to conclude that S. pseudintermedius isolates obtained from clinical samples in dogs from Campo Grande-MS are characterized by high resistance to antimicrobials, but absence of methicillin-resistant Staphylococcus pseudintermedius.
DEVELOPMENT AGENCY: Fundação Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Universidade Federal de Mato Grosso do Sul (UFMS). Palavras-chave: Animal infection, molecular diagnosis, one health, resistance, surveillance Agência de fomento:Fundação Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |||||